Journal:

Article Title: Expression of Simian Immunodeficiency Virus nef in Immune Cells of Transgenic Mice Leads to a Severe AIDS-Like Disease

doi: 10.1128/JVI.76.8.3981-3995.2002

Figure Lengend Snippet: Expression of SIVmac239 Nef protein in CD4C/SHIV-nefSIV Tg mice. (A) Total protein extracts (100 μg) from thymuses of 1-month-old Tg lines (from founders F57691, F55541, and F57693) and non-Tg (nTg) littermates were separated by SDS-polyacrylamide gel electrophoresis and analyzed by Western blotting with a monoclonal antibody specific to SIV Nef. Equal loading was confirmed by probing with an anti-actin antibody. (B) Semiquantitative analysis of SIVmac239 Nef protein. Thymic extracts from mice from Tg lines were serially diluted and compared to each other for the level of Nef expression. Relative amounts of protein are shown above lanes; the well indicated by “4×” contains four times as much protein as the well indicated by “1×”. (C) Analysis of Nef expression in specific cell populations. CD4+ TcRαβ+ (CD4+) T cells and CD8+ TcRαβ+ (CD8+) T cells from spleens (Spl.) or peripheral (p) or mesenteric (m) LN, B220+ IgM+ B cells from spleens, double-positive CD4+ CD8+ thymocytes (thymus), and peritoneal macrophages (Mφ) from Tg and non-Tg mice were obtained by cell sorting (or plating in the case of macrophages), and cell extracts were processed for Western blotting with an anti-Nef monoclonal antibody. Blots were stripped of bound antibodies and reprobed with anti-actin antibodies to assess the amount of loaded protein.

Article Snippet: Staining was also performed with a PE-coupled anti-mouse B220 (Ly5) monoclonal antibody and an FITC-coupled anti-mouse IgM monoclonal antibody (both from Cedarlane).

Techniques: Expressing, Polyacrylamide Gel Electrophoresis, Western Blot, FACS

Journal: Infection and Immunity

Article Title: Characterization of Oral Yersinia enterocolitica Infection in Three Different Strains of Inbred Mice

doi: 10.1128/iai.72.3.1645-1656.2004

Figure Lengend Snippet: FIG. 7. Immunophenotyping. T-cell populations and B cells from C57BL/6j (I), BALB/cj (F), and 129X1/Svj (Œ) tissues following oral infection with Y. enterocolitica are shown. Values on y axis indicate the percentage of cells from the lymphocyte gate (TCR, mature T-cells; CD3-CD62L, activated T cells; CD-19, B cells) or ratios of these values (CD4, Th cells; CD8, cytotoxic T cells).

Article Snippet: The following Abs and secondary staining reagents were used for flow cytometric studies: biotin-conjugated rat anti-mouse Ly-6G (Gr-1: RB6-8C5), biotinconjugated hamster anti-mouse T-cell receptor (TCR) chain (H57-597), PE-conjugated rat anti-mouse CD19 (1D3), FITC-conjugated rat anti-mouse LY-49G2 (4D11), PE-conjugated hamster anti-mouse CD11c (HL3), PE-conjugated hamster anti-mouse T-cell receptor (GL3), FITC-conjugated rat antimouse CD4 (RM4-5), FITC-conjugated rat anti-mouse CD8a (53-6.7), PE-conjugated rat anti-mouse CD25 (PC61), PE-conjugated rat anti-mouse CD62L (MEL-14), FITC-conjugated hamster anti-mouse CD3ε chain (145-2C11), and appropriate isotype control Abs, streptavidin-FITC, streptavidin-PE (all from BD Pharmingen), and biotin-conjugated rat anti-mouse BM8 (Novus Biologicals, Littleton, Colo.).

Techniques: Infection